Sperm Retrieval from Testis (PESA/TESE)

An option for men who have no sperm detected in semen analysis or have a blockage in the sperm duct. Sperm is retrieved directly from the epididymis or testicular tissue and used in conjunction with ICSI treatment.

About

What is PESA/TESE?

Sperm retrieval from the testis is a procedure in which the doctor uses a fine needle or a minor surgical technique to collect sperm from the epididymis or testicular tissue. The sample is then processed in a laboratory to identify viable sperm.
The selected sperm is used with the female partner’s eggs through ICSI, a technique that enables fertilization even in cases with very low sperm count or no sperm found in semen.

PESA / TESE

A specialized sperm retrieval technique for male infertility cases where no sperm is found in semen, but sperm may still be present in the epididymis or testicular tissue.

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    Used in combination with ICSI

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    Suitable for men with no sperm detected in semen analysis

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    Performed by specialized medical and laboratory teams

Techniques

Difference between PESA and TESE

Both procedures aim to retrieve sperm directly from the male reproductive system. However, the location and method of collection differ depending on the doctor’s evaluation.

PESA

Percutaneous Epididymal Sperm Aspiration

A fine needle is used to pass through the skin of the scrotum into the epididymal tube to aspirate sperm. The procedure typically takes about 10–20 minutes, and patients can usually rest for around 1 hour before returning home.

TESE

Testicular Sperm Extraction

A small surgical procedure is performed to remove a tiny sample of testicular tissue, which is then examined under a microscope to retrieve sperm. The procedure is usually done under anesthesia, and mild pain or discomfort may occur afterward, typically improving within a few days.

How It Works

What happens after sperm retrieval?

After the sample is collected, embryologists transfer it to a standardized laboratory to identify and select viable sperm. The sperm is then used in conjunction with the ICSI procedure.
This process helps improve the chances of successful fertility treatment in cases where no sperm is found in the semen, while also considering egg quality and the overall treatment plan of the female partner.

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    Doctor evaluates the cause of azoospermia (no sperm found in semen)

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    Selects the appropriate method: PESA or TESE

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    Retrieves sperm from the epididymis or testicular tissue

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    Sperm is identified and selected in the laboratory

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    Viable sperm is used in the ICSI procedure

Who Should Consider

Who is PESA/TESE suitable for?

Doctors may recommend PESA or TESE in cases where no sperm is found in the semen analysis, or when sperm cannot be released through the ejaculate due to underlying conditions.

Male infertility caused by blocked sperm ducts

Congenital absence of the vas deferens

Men who have undergone vasectomy and later wish to have children

Obstruction caused by infection or previous surgery
No sperm detected in routine semen analysis
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Benefits

Advantages of Direct Sperm Retrieval from the Testis

Expands options in cases with no sperm detected

Helps retrieve sperm from the epididymis or testicular tissue even when no sperm is found in the semen.

Can be used with ICSI

Retrieved sperm can be used with ICSI to achieve fertilization with the egg.

Suitable for duct-related problems

An option for men with blocked sperm ducts, congenital absence of the vas deferens, or previous vasectomy.

Personalized treatment planning

The doctor selects the most appropriate method based on the underlying cause, medical history, and test results of the male partner.

Medical Note

Which is better PESA or TESE?

The choice between PESA and TESE depends on the cause of azoospermia, the type of blockage, semen analysis results, and evaluation by a fertility specialist. In some cases, a less invasive method may be tried first, while in others, direct testicular sperm retrieval may be more appropriate.

Start with Confidence

Consult PESA/TESE Sperm Retrieval

If no sperm is found in semen analysis or there is a history of blocked sperm ducts, you can consult Gift Fertility Centre Bangkok for a proper evaluation and personalized treatment plan.

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